الفهرس 
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Abstract
The genus Aeromonas consists of ubiquitous gram-negative rods that are widely distributed. The organism is frequently isolated from retail produce sources and meat products. A total number of 302 red meat samples fresh and frozen were colleted from cattle (82), buffalo (149), sheep (31) and camel (40) from different parts of the carcass. Out of the 302 samples of fresh and frozen meat, a total of 17 samples were found to harbor Aeromonas species with an incidence of 5.6%. A. hyDROPhila was the most frequently isolated serotype from fresh in percentage of 4.2% and from frozen meat in a percentage of 2.2%. This was followed by A. caviae which was isolated from fresh in percentage of 0.6% and frozen meat in a percentage of 2.2%. The incidence of isolation of A. sobria which was isolated from fresh 1.2% and frozen meat at a rate of 0.7%. Using the s-PCR, 17 isolates of Aeromonas species showed amplification product at 550 bp fragment characteristic for genus Aeromonas. The d-PCR assay allowed the amplification product for genus and species specific fragments of DNA from 10 isolates at 550 bp and 700 bp fragment characteristic for A. hyDROPhila. The amplification product at 320 bp and 1170 bp fragments characteristic for 4 local isolates of A. ca viae, 3 local isolates of A. sobria respectively. 17 isolates of Aeromonas species were studied for the amplification of virulence genes [hemolysin and aerolysin genes of A. hyDROPhila and hemolysin gene of A. sobria. The amplification of the aerolysin gene from 3 isolates of A. hyDROPhila was at 309 bp. The aerolysin gene was not detected in the A. caviae nor from A. sobria. The hemolysin gene was not detected in any of the 17 isolates.