الفهرس | Only 14 pages are availabe for public view |
Abstract Depending on preparation of hyperimmune serum of L. monocytogenes standard strain serotype 4b in rabbit . The present study evaluated the ability to isolate Listeria from foods, using shortened procedure of sample enrichment followed by immunomagnetic separation or filtration methods, and serological identification of Listeria was achieved in much shorter time (40-48h)than with classical cultivation and biochemical identification procedures. The rapid methods such as PHA, ELISA, Dot ELISA and Western blot are easy to perform, their specificity is very high, fulfills the expectations and can detect listeria in food samples containing not too many Listeria (1-10 CFU/25g). Also colony blot methods necessary to distinguish L. monocytogenes from other Listeria species and some Gram negative bacteria as E. coli, C. jejuni and S. Typhimurium. |