الفهرس Only 14 pages are availabe for public view
 |  | from | 126 |  |  |
| | | from | 126 | | |
Abstract
A total of 120 random samples of meat products (beef burger, beef luncheon and Oriental sausage) and chicken products (chicken fillet, chicken luncheon and chicken thigh), (20 of each) were collected from different supermarkets at El-Kalyobia and El-Menofia governorates, the collected samples were examined bacteriologically for detection of E.coli strains especially enterohaemorrhagic E.coli, then confirmed by recent technique as PCR the obtained results indicated that E. coli was isolated from 10% and 5% of the examined beef burger and oriental sausage samples, respectively, However, all beef luncheon samples were free from E.coli, Moreover, all examined samples found to be free from E.coli O157 : H7, the isolated E.coli serotypes were O111:K58 (B9) and O119 : K69 (B12) from beef burger and O86:K61 (B7) from oriental sausage, So, the incidence of Enterohaemorrhagic E.coli was 5% in beef burger the obtained results indicated that E. coli was isolated from 15% and 35% of the examined chicken fillet and chicken thigh, respectively, all chicken luncheon samples were free from E.coli. Moreover, all examined samples found to be free from E.coli O157 : H7, the isolated E.coli serotypes were O26 : K60 (B6), O111:K58 (B9) and O128 : K67 (B12) in chicken fillet and O26:K60 (B6), O55 : K59(B5), O111 : K58 (B9) and O124 : K72(B17) in chicken thigh, this indicates that incidence of Enterohaemorrhagic E.coli was 10%, 25% in chicken fillet and chicken thigh, respectively more over, the results cleared that PCR is an ideal method for identification of E. coli, as it was effective, less labor, more sensitive, reduces effort and time, the pathogenicity of EHEC is associated with several virulence factors, including VT1 and VT2, therefore, real time PCR targets for detection of both of them, it was found that not all EHEC are positive for both VT1 and VT2, as E. coli O111 : K58 (B9) is positive for VT1 and VT2, but O26 : K60 (B6) is only positive for VT1, the public health significance of the isolated Enteropathogenic E.coli from meat and poultry products and probable sources of their contamination as well as some recommendations to control such serious pathogens were discussed.