الفهرس Only 14 pages are availabe for public view
 |  | from | 149 |  |  |
| | | from | 149 | | |
Abstract
The present study aims to investigate the influence of endotoxin (ET)
treatment on adult male albino rats, as well as, to shed light on the protective
role of propolis (Prop) for modulating the adverse effects in some
neurological and immunological parameters associated with ET treatment.
Seventy eight rats weighing 100-150g were divided into four groups. The
first served as control group (6 rats) and were received a daily intraperitoneal
(i.p) injection of saline solution (0.9% NaCI) for 15 consecutive days. The
second group (24 rats); were received an i.p injection of 0.9% NaCI for 15
consecutive days, then received i.p injection of ET (lmg/kg/day) at the 16th
day of experiment for 4 repeated days. The third group (24 rats); were
received an i.p injection of Prop (l50mg/kg/day) for 19 consecutive days.
The fourth group (24 rats); were received the same route of Prop
administration as mentioned in the third group; then, the rats were received
ET as described in the second group. Animals of all groups were decapitated
2 hours post-treatment at 16th, 1 i\ 18th and 19th days of experiment. Changes
in norepinephrine (NE), dopamine (DA), serotonin (5-HT) and nitric oxide
(NO) levels in cerebral cortex were measured. Also, spleen cellularity and
viability were assessed. In addition, the proliferative response of spleen
lymphocytes to both lipopolysaccharide (LPS) and phytohaemaglutinin
(PHA) mitogens was detected. Moreover i.p macrophages were counted and
their NO production was measured in all groups. The results indicated that,
injection of ET to white male albino rats induced a dramatic change in
monoaminergic system in cerebral cortex and caused an increase in NO
production in cerebral cortex as well as in i.p macrophage. Moreover, ET
induced a potential immunostimulation for splenocytes and augmented the
viability of peritoneal macrophages. It could be concluded from this study
that the treatment with Prop has antioxidant; antibacterial and anti-
inflammatory properties which could abolish the disturbance in neural and
immune systems induced by ET treatment.