الفهرس | Only 14 pages are availabe for public view |
Abstract The present study included 34 cases of newly diagnoses acute lymphoblastic leukemia. Fifteen cases were lost due to contamination and other 2 cases failed to show neither metaphase nor interphase nuclei. The remaining 17 cases were successfully processed. They were found to be of c-ALL (7cases 41.1%), pre B-ALL (8 cases 47%) and T-ALL (2 cases 11.7%) phenotypes by both morphology and immunopheotyping. They were further subjected to karyotyping after direct, synchronization and short term blood culture techniques using GTG banding method. Fluorescent in situ hybridization analysis was done afterwards using red/green labeled break apart dual colour probes spanning the critical region 11q23. A single case revealed successful cytogenetics but with fuzzy bad morphology and overcrowded metaphase spread chromosomes. FISH results revealed a single case with (75% positivity of its counted nuclei) with translocation of one chromosome 11q23, while double translocations involving chromosome 11q23 were found in (10% counted nuclei) and a deletion of one chromosome 11q23 (5%) seen in one nucleus. The remaining nuclei (5%) showed normal chromosomes 11 without any rearrangements detected. Twelve cases showed from 5-15% positive t(11q23); case 1 showed double t(11q23) in one nucleus and single translocation in another nucleus. There was one cell with complete chromosome 11 deletion, 2 minimal probe separation were once recorded, while the remaining cells were negative for any chromosome 11 abnormality. Case 2 had one positively counted translocation, minimal probe separation was seen in other 3 nuclei while the remaining cells were found to be normal with no apparent chromosome 11 rearrangement. Case 4 had only 2 minimally separated signals. Case 5 had 3 positive translocations of one chromosome 11, one nucleus with double t(11q23) and one minimally separated red/green probes. Case 7 had 3 nuclei showing deletion of one chromosome 11 and 2 minimally separated probe signals. Case 8 showed one split apart signal, one minimally separated signal and one deleted signal. Case 9 had 2 nuclei with positive translocations of one chromosome 11, one nucleus with double probe separation, one nucleus with double minimally separated probes and another nucleus with a single minimal probe separation. Case 11 had 3 nuclei with positive chromosome 11 translocation, a minimally separated signal seen in other 3 nuclei, double minimally separated probes detected in another nucleus and a deleted chromosome 11 seen in another nucleus. Case 13 and 16 had one positive signal splitting in one nucleus and another rminimally separated signal. Case 14 revealed 2 nuclei with positive split signal and one minimal probe separation. Case 15 had 2 nuclei with a deleted chromosome 11 in each one and a single minimal probe separation. Four cases were found to be lacking any chromosome 11q23 translocations and were considered as negative FISH results. Six cases(1,7,8,10,11,15) showed either complete or partial deletion of one chromosome 11. Five cases showed translocations involving both chromosomes 11 cases (1,5,9,10,16) that was not reported in the literature before. |