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Abstract Tie neck rot disease, caused by Botryt allii Munn. js one of the most serious ciseases that attack onion aants grown for seed or bulb production and bulbs during storage or transit. Theroore, the present investiation was planned to study the effect of expsing onion ‘bulb crop p1ant to certain cultural practices i.e. irrigation, rtilization, sowing spacing and curing process on SuS— eptibility of onion plants raised from resulting bulbs F to neck rot disease under :[ie].d conditions. Influence of some factors on incidence of neck rot disease of oiion under :reenhouse conditions and role of certain enzyi:ies in virulence of Botrytis allii isolates wre also inveEti— gated. Results of this work can be summarized as follows: 1) Fourteen isolates of Botrytis allii Munn. were isolated from diseased onion plants collected from certains Governorates of Upper Egypt. All isolates were pat’a:eric to seed crop plants of Giza S and Behairi cultivars. 2) Virulence of Botr;tis allii isolates varied from highlï pathogenic to moderately pathogenic or weakly pathojenic. 3) Giza 6 cultivar waS more susceptible to the disease than 3ehairi cultivar under ll factors ts1ed in this Study. |