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Abstract The present work was planned to study the possibility of biocontrol of certain diseases caused by the soil-borne scierotial fungi Macrophomina phaseolina, Rhizoctonia,solani, Sclerotinia sclerotioruzn, Sclerotiuzn cepivorum and Salerotium rolfsii. Isolation of several antagonists from soil and dead scierotia and testing their effect on growth of the pathogenic fungi were conducted. Effect of antagonists on formation and viability of sclerotia of the pathogens, disease incidence as well as plant growth and seed germination of hosts was also investigated throughout this work. The results could be summarized in the following: I - Ten isolates of M. phaseolina, R. solani, S. sclerotiorum, S. cepivorum and s. rolfsii were isolated from naturally infected plants of sesame (sciero— tial wilt),cotton (soreshin), sunflower (root rot and wilt disease complex), onion (white rot) and sugar beet (root rot), respectively. 2 — Testing pathogenicity of s. cepivorum isolates on 11Giza 6 onion cultivar and pathogenicity of other fungal isolates on 11Dandara” cotton cultivar, Giza 32” sesame cultivar, “Einback” sugar beet cultivar, and “Maik” sunflower cultivar revealed that the fungal isolates were pathogenic on the tested cultivars. S. cepivoruin caused white rot on |