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Abstract
The water buffalo is considered an essential component of the Egyptian animal
wealth. They produce about 60% of totally produced milk and about 40% of the
totally produced meat in Egypt. So, water buffalo becomes central to the Egyptian
farm animals and the Egyptian government tries hard on controlling the infectious
agents among such species by the application of control programs based primarily
on immunological aspects.
One of the major difficulties for the application of such control programs was
the slow progress in elucidating the mechanisms regulating the immune response to
the infectious agents and their derived vaccines. This was the result of lack of mAbs
reagents needed for studying the immune system and immune response in water
buffalo. So, the aim of the present study was to provide an insight toward the
immune system of water buffalo using mAbs and flow cytometry technology. This
was achieved by;
1- Screening of a large set of mAbs that recognize bovine MHC and leukocyte
differentiation molecules to identify mAbs that react with orthologous molecules
in water buffalo. It was found that analysis of ninety mAbs developed against
leukocyte differentiation molecules in cattle, sheep, goats, lama, pigs, dogs and
human revealed that these mAbs recognize conserved epitopes on orthologous
leukocyte differentiation molecules in water buffalo. This includes mAbs
specific for MHC class I, MHC class II, CD2, CD3, CD4, CD6, CD8, CD11a,
CD11b, CD11c, CD14, CD18, CD25, CD44, CD45R, CD62L, CD172a. Also
mAbs that recognize γδ TCR, WC1 molecules expressed on activated
lymphocytes were identified