الفهرس | Only 14 pages are availabe for public view |
Abstract Urinary tract infection is a common bacterial infection occurring during pregnancy, beginning in week 6 and peaking during weeks 22–24 of pregnancy. Asymptomatic bacteriuria is a major risk factor for the development of urinary tract infection. If not properly treated, it may lead to several maternal and foetal complications. Although several different microorganisms can cause UTIs,including fungi and viruses, bacteria are the major causative organisms and are responsible for more than 95% of UTI cases. E. coli are the microorganisms most commonly isolated from patients with complicated as well as uncomplicated urinary tract infections. The aim of this work was screening for asymptomatic bacteriuria during pregnancy by quantitative urine culture (bacterial cell count) and other screening tests, determining the prevalence of uropathogens causing symptomatic and asymptomatic UTI in pregnant women, detecting susceptibility of bacterial isolates against different antimicrobial agents and evaluating the diagnostic efficacy of various screening tests in detecting asymptomatic bacteriuria among pregnant women. In this study subjects were classified into two groups: Group 1: Included 150 pregnant females who were attending Obstetric and Gynecology clinic at Menofyia University Hospitals or Maternity and Child Health Center of Shebin El-Kom city asking for antenatal care. They were distributed as 50 pregnant females in each of the three trimesters. Group 2: Included 150 non-pregnant healthy females matched by age as a control group. All urine samples were subjected to four rapid screening tests(dipstick biochemical analysis of urine for nitrites and leukocyte esterase,as well as microscopic examination of urine for pus cells and bacteria). The obtained results were compared with that of urine culture to evaluate the validity of these tests for screening for asymptomatic bacteriuria in pregnant females and for early detection of urinary tract infection in non pregnant females. Isolation and identification of the causative organisms were done according to the standard microbiological methods. In addition, all Gram negative isolates were identified by the API 20E biochemical test kit(Biomerieux). MICs of vancomycin were determined for all staphylococcal and enterococcal isolates. Different isolated planktonic bacteria were tested for In vitro adherence to plastic tissue plates to examine biofilm forming capacity of these isolates. |