الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
The present study evaluated ‘in vitro’ the antimicrobial efficacy of photo activated disinfection against Enterococcus faecalis infecting the root canals in comparison with 2.5% Sodium hypochlorite and 2% Chlorhexidine.
Sixty single rooted teeth were selected for the study. After decoronation, the working length was determined and the root canals were prepared up to size F3 protper rotary instrument in crown down technique. The teeth were sterilized and the apical foramina were sealed with glass ionomer cement and the teeth were coated with two layers of nail varnish. 10 µl of Enterococcus faecalis suspension adjust optically for 0.5 McFarland were inoculated for each root canal. The teeth were incubated at 37 0C for seven days for bacterial habilitation. Then the teeth were divided randomly into 4 groups each of 15 teeth according to the method of disinfection as follows:
Group I: Photo-activated disinfection (PAD)
Group II: Sodium hypochlorite (NaOCl) 2.5%
Group III: Chlorhexidine 2% (CHX)
Group IV: control
Three bacterial samples of the infected root canals were collected throughout the procedure, the first sample was collected before the treatment(S1) , the second sample was collected immediately after treatment(S2), and the third one(S3) was collected 10 days later. The samples were inoculated onto the blood agar plates and the colony forming units (CFU) were counted.
The results from the culture immediate after treatment showed that the PAD has the highest microbial reduction followed by 2.5% NaOCl, then 2% Chlorhexidine. After 10 days of treatment 2% Chlorhexidine revealed the highest antimicrobial effect.
from the present study, the following conclusions can be drawn:
1- The Photo-activated disinfection (PAD) is an effective method for immediate disinfection of the root canals infected with Enterococcus faecalis.
2- 2% Chlorhexidine showed the longer sustained antimicrobial efficacy against Enterococcus faecalis more than PAD and 2.5% NaOCl.