الفهرس | Only 14 pages are availabe for public view |
Abstract Rotaviruses are a major cause of acute viral gastroenteritis in infants and children but can cause gastroenteritis in all age groups. In the present study, 168 sewage samples were collected during two years (2006-2008) from two wastewater treatment plants (Zenin and EI Gabal El Asfar). Wastewater samples were collected from inlet, after primary sedimentation, after secondary sedimentation and chlorinated effluent of the examined WWTPs. Samples were concentrated by filtration through nitrocellulose membrane (0.45~m pore size and 142 mm diameter) and re-concentrated by organic flocculation method for detection of rotavirus. Also 567 stool samples were collected from hospitalized children in Said Galal hospital, Bolak hospital, Abo El Rish hospital during one year period of this study. Examination of stool and wastewater samples were carried out by using RT-PCR to detect rotavirus geno~es then confirmed by sequencing. G and P typing was carried out by multiplex nested PCR and multiplex semi-nested PCR. The result showed that the incidence of rotavirus were 20.8% and 28.6% in raw wastewater and stool samples, respectively. The genotypes of rotavirus in stool samples were G I P[ 4j, G3P[ 4], GIP[6] and G3P[6] in percentages of 14.8%, 9.9%, 9.9% and 8.60/0, respectively. On the other hand rotavirus genotypes in wastewater samples were G IP[ 4] and G3P[ 4], in percentages of 20.6 % and 5.9%, respectively. |