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Abstract
Mastitis is a serious problem in dairy farms not only for the associated economic losses but also because of its hazards of zoonotic diseases. S. aureus is considered one of the most important and widespread pathogens causing mastitis. Therefore, the current study was undertaken to isolate S. aureus from clinical mastitis milk collected from two dairy cattle farms in Bohera governorate.
The results showed that the incidence of clinical mastitis was 20.8% among 1000 Holstein-Friesian cows raised in two dairy herds based on the changes in milk characteristics.
Bacteriological examination of 208 clinical mastitis milk samples yielded 65 isolates with an incidence of 31.25% were suspected to be staphylococci since these isolates showed pigmentation characteristics and were Gram-positive cocci.
Twenty-two isolates with an incidence of 33.85% out of the 65 suspected isolates were positive for coagulase activity using the tube coagulase test (TCT).
Results showed the ability of TCT on the isolates to detect the presence of pathogens. The test is inexpensive and easy to perform with limited laboratory facilities.
PCR technique was used as confirmatory, rapid, sensitive and specific tool to detect S. aureus enterotoxin A (SEA) coding gene in eight S. aureus bacteriologically positive samples. The technique used was PCR using specific primers for S. aureus SEA, which is one of the pathogenic determinants of S. aureus isolated from bovine milk. Two isolates were positive for SEA coding gene.
In consideration of time and money consumed in traditional bacteriological examination methods and PCR techniques, the results confirm the ability of TCT for detection of the pathogenicity of S. aureus as a simple, non-expensive and easy to perform system.