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العنوان
Genetic and cytological studies on plant tissue culture under specific conditions /
المؤلف
Rhizakla, A.A.
هيئة الاعداد
باحث / A.A. Rhizakla
مشرف / H.R. Nazeem
مناقش / A.M. Hassan
مناقش / H.R. Nazeem
الموضوع
Plant cell culture. Plant tissue culture. Plant breeding. Botany, Economic Plant micropropagation. Germplasm resources, Plant. Crops Genetic engineering.
تاريخ النشر
1992.
عدد الصفحات
158 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/1992
مكان الإجازة
جامعة بنها - كلية الزراعة - وراثة
الفهرس
Only 14 pages are availabe for public view

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Abstract

SUMMARY
The present study aimed to study the relationship
between plant tissues in different media, calli growth
behaviour and their proliferai ton with their genetical
and cytological behaviour using three ~ mays cultivars;
one local; Giza 2 (G2), and two American; PI 451690
and 451694.
The results obtained could iDe summarized as follows:
1- The Egyptian cultivar G2 hypocotyl explants grown
on MS medium supplemented with 10 mg 1M/I, gave
quite different growth behavi.our.
2- The cytological examination for this group of the
hypocotyls, which were able to differentiate on
this medium, i.•e., MS + 10 mg lAA/l showed mitotic
index reached to 38.6% which is higher than that
for the control plants which reached to 23 .•6%-
3- Use of different media, gave different growth
behaviour and differentiation.. In many instances,
the mitotic index diminished to a greater extent.
4- The cytological investigations showed many types
of chromosomal abnormalities in the different media
supplemented with different hormones •

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5- Similar results were obtained using
explants, e.g., root tips for instance.
different
6- Seeds growth on MS medium supplemented with different
concentrations of 2,4_0 showed big differences in
growth behaviour and cytological investigations.
7- The mitotic index was reduced to a great extent
with wide variations in chromosomal aberrations.
8- The mature embryos did not
N6 medium supplemented with
2,4_0/1 and 25 roM L. proline.
show any response on
either 1 mg or 5 mg
9- The mature embryos on N6 medium supplemented with
10 mg IAA/1 and 1 kg Kin/1 gave only one survived
embryo showing four thin roots and a stem with one
abnormal leaf. The cytological examinations showed
large number of nucleoli whether outside or inside
the nucleus.
10- Different results for growth behaviour and cytological
examination, from those of G2, were obtained for
the cultivar PI 451690. Also the American cultivar
PI 451694 gave different behaviour for growth and
cell divisions with various types of chromosomal
aberrations.