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Abstract This study was carried out during 1980-1981 at the Poultry Experiment Station, Department of Animal Production, Faculty of Agriculture Zagazig University, Benhabranch. Biochemical and radio immunological.assays were perf ormed in the laboratories of the Radioimmunologyand Biochemistry Unit, Nuclear Research Centre, Atomic Energy Establishment. The purpose of this study was: 1- Estimation of progesterone, thyroxine, triiodothyronine levels and somebiochemical parameters in blood serum of F~oumi and RhodeIsland Red l~1Dg pullets during the time of egg formation. 2 - The variation in progesterone, thyroxine, triiodothyronine levels and someblood serum parameters in ~ayoum1and BIR breeds due to the different clutch size. 3 - The relationship between serum progesterone levels and the thyro;Line, triiodothyronine, total protein, albumin, globulin, calcium, inorganic phosphorus and &”’t cholesterol levelslovulat:1.on time in J’ayoWDiand RIR pullets. - 135 - The study was performed on 60 Fayoumi and 40 RIR laying pullets from a flock of 500 chickens. hatched in March 1980. exposed to natural day light and fed .AS lib. well balanced diet along the experimental period. The selected pullets were of different clutch size and regular clutch sequences. Time of oviposition was recorded for each pullets to obtain the approximate time of ovulation. Time between OViposition and ovulation was considered to be )0 minutes Blood samples were collected from the wing vain. usdng hepari..nized siringes at ovulation time 2 and 4 hours after oVulation.-20 - 16 - 10 - 8 - 6 - 4 - 2 hra. before the expected ovulation of the second ovum. Radioimmunoassay-has been used for the determination of the hormone levels in pullet s serum. The obtained findings are sIlIDIoarized.1n the follOWings: 1 - ~!!’!!!!LE..2”28!!!!~!!!: The serum progesterone level was 45 % higher in the RIR breed than. in F~own1 ones. It ranges from 0.041 to 0.61 ng/ml and from 0.11 to 0.86 ng/ml for Feyoum1and RIR breed. respectively. ----_._-- - 136 - Constant and gradual increase in serum progesterone level was evident with the progress of time towards the ovulation reaching its peak value at -4 hra for both breeds and then decreased gradually there after. As a function of breed serum progesterone level in RIR was found to be higher than Fayoumibreed at s:ny interval of estimation. The progesterone level in RIR is correlated with the clutch size, while in the Fay-oumit showeda fluctuating pattern increased at 3 eggs and lowered minimal value at 5 eggs. The serum total thyroxine and serum triiodothyronine levels are higher in RIR than the corresponding mean values in Fayoumiby 56.5 % and 12. J % for T4 and T) respectively. The calculated T)fT 4 ratio was lower in the RIR breed than the Fayoumi breed. No consistent pattern was found for any of the formar prameters with the progress of time towards ovulation, also relatively high mean value for T4 was evident to -6 hra. prior to ovulation in both breed. The Fayoumi hens lilio.ed a decreasing T4 values with the increase in clutch size, while the RIRbreed showedan increasing trend. · - - - - - 13’1 - On the other hand. T 3 levels is correlated with the clutch size in RIR breed and is negatively corre1e. ted in Fe.youmibreed starting from clutch siZe 3 to 5 eggs with relatively lower value for clutch size 2. T 3 /T 4 ratio in Fe.youmibreed are positivelY correlated with the clutoh size and negatively correlated in RIR breed. An increasing trend for T3 waS shown in RIRbreed as the progesterone level increase. whUe T4 fluctuate with the increase of progesterone categories showing an increasing followed by a decreasing trendS. The obser\’ed increase in serum toteJ. protein and serum eJ.bUJJrlnin laying pu1lets are hig)J.1ycorrelated with the progesterone level during egg forua tion • There is a slight increase in serum toteJ. protein with the increese in clutch size in both Fe.youmiand RIR breed. Similar trend wes noted in serum eJ.bUJJrlnin Fe.youmi but not in the RIll. Serwn globu1in showedno appearent trend in Fe.youmi breed. while it is correlated with the clutch size in the RIRbreed. - 138 - No considerable change 1n the A/G ratio was shown with the ~crease in clutch size in both breed. With the increase in serum progesterone there was an increase in serum total protein, albumin and globulin demonestrated by the two breeds. However, no considerable changes were noted for A/G ratio. As a function of egg formation intervals, serum calcium level was at its maximumconcentration at -20 hra before ovulation in Fayoumi breed and 0 hr. in RIR breed. However, its lowest concentration was noted at +2 hours after ovulation in both breeds. On the other hand inorganic phosphorus showed its high level at -2 and -10 for RIR and Fayoumi respectively. As far as the clutch size is conserned, calcium and inorganic phosphorus levels increase in the large clutch size, while CalIF ratio showed a decreasing trends. Calcium does not follow the course of progesterone as its values remain constant or fluctuate in a narrow range with the increase of progesterone categories in RIR and Fayoumi breeds respectively. However, phosphorus content shewed a slight increasing trend. ~-~----~-~~ ~~~~~-----------------~._------- - 139 - The highest Ca/IP ratio values was attained at 0 and -20 hours for Fayoumi and RIR respectively t while no single low value was deducted from both breeds. 5 - S-e-r-u--m--c-h-o-l-e-s-t-e-r-o-l: Serum cholesterol levels in Fayoumi exceeds those in RIR breed, although the differences was statistically significants. No consistent pattern was noted for serum cholesterol in relation to the time of ovulation for both breeds. A consistant gradual increasing ~rend was observed with the increase of clutch size in Fayoumi hens, while in RIR it begins at clutch size 3. Cholesterol is positevely correlated to progesterone categories in F8\Youmi breed and not in the RIR breed in the lower progesteron categories. 6 - Q2!!!~!!~2~_22!!~!2!~!_~2~!!~_!~~!_R!2g~!1!!2~_!!!!! ~~_2!B!!!~2!!_!:!:!l!_~~_2!!!!!r_!!!g2g_P!!.”!!!!!:r!..f. - .~. - . . . =- Correlation coefficient values between serum progesterone level at ovulation time and each of blood parameters studied differed due to breed. While the correlation ~ coefficient showed significant (p <0.05) possitive values - 140 - between serum progesterone level and each of total prote~ and albumin in Fayoumi. correlation coefficient between serum progesterone and each of thyrox~e, total protein, albumin, globulin and inorganic phosphorus showed significant (p < O.01) pOBs1tive values and significant (p (0.01) negative value between progesterone and triiodothyronine in Phode Island Red. |