الفهرس 
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Abstract
The aim of the present work is to study the effect of ozone therapy on DNA of tongue in rats Using :
1) Histological study (H&E).
2) Molecular study of DNA using RAPD-PCR.
CONCLUSSION
In the view of the previous results and discussion it can be concluded that :
1. There is an increase in the keratinized layer of the epithelium with the increase in the dose and duration of ozone administration. There is increase in the orthokeratin in some areas and in other areas the keratin appeared as a network of parakeratinous layer which gives this layer an abnormal histological appearance in the posterior part.
2. There is an increase in the epithelial thickness (acanthosis) with increased intercellular spaces and increased vaculzation.
3. The connective tissue stroma underlying the epithelium shows increased vascularity (vasodilatation) with increased number of fibroblast and inflammatory cells infilteration.
4. The collagen fibers content increase in number and thickness and become very thick confined to the papillae.
5. The result of DNA study showed that the quality and quantity of the DNA of the treated groups increased and adavanced in proprotional to the doses and duration of ozone application and the previous DNA primers assur these results.
6. Molecular study showed that there is increase in the optical density and DNA bands in proprotional to the increase in the doses and duration of ozone application .
7. RAPD-PCR analysis is a good and specific diagnostic tool to study the molecular effect of drugs such as ozone.