الفهرس 
Materials and methodsOnly 14 pages are availabe for public view
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Abstract
The present study began with 90 adult albino rats of both sexes (60 females and 30 males). The animals were divided into 4 major groups, the first group served as control group comprising 36 rats of both sexes and 3 experimental groups each comprised 18 rats of both sexes. The control group was further divided into 2 subgroups each containing 18 rats as follows.
-Ve control animals received solvents of both diabetogenic drug and vitamin E equivalent to the volume used in experimental animals throughout the study.
The second subgroup (+ ye control animals) received single dose of 40 mg/kg of streptozotocin and subcutaneous injection with olive oil solvent of vit. E
The second group (experimental): both sexes were injected intraperitonealy with freshly prepared aqueous solution of streptozotocin (diabetogenic) in a single dose of 40 mg/kg body weight one week before mating.
Animals of the third group received vitamin E in a dose of 400 mg/day s.c. 2 weeks before artificially induced diabetes with streptozotocin.
The fourth group comprised animals receiving streptozotocin drug one week before mating and vitamin E injection one week after pregnancy and continued until the day of delivery.
The offspring of each group were examined for diabetes after 2(a) and 4 (b) weeks the latter were subdivided into subgroup b 1 (diabetic), b2 (non diabetic) according to the presence of diabetes.
For each animal, biochemical investigation (blood glucose concentration measurement), histological study (morphological), quantitative study (statistical analysis of the volumetric data according to t distribution test), and finally chromosomal study (direct metaphase from bone marrow cells) were done.
The results showed that:
1. parent animals (adult) of all groups except GIA (- ye control) showed an increased blood glucose level (diabetic) due to streptozotocin injection.
•At the age of 2 weeks offspring of diabetic mothers there was hypoglycemia.
•In 4 weeks offspring of GIBb, Glib GIIIb and GIVb definite hyperglycemia (blood glucose level < 200mg/d1) was present, and was significant (p<0.005) in GII and significant (p<0.01) in GIV.
•The blood glucose level was directly proportional to the histological (morphological) and quantitative changes concerning n-cells. 13-cells showed degenerative changes in the form of pyknoiss, karyorrhexis, ballooning of their cytoplasm (hyDROPic degeneration) and lastly complete cell loss.
•These histological changes were associated with increased blood glucose concentration and decreased percentage of 13-cells.
•These changes were present in adult animals (parents) and in 4-weeks offspring (diabetic).
•In 2- weeks offspring, the (3-cell percentages were higher than normal (hyperplasia) and this was corresponding to the hypoglycemic state present in this age.
•There was highly significant increase in B-cell percentage in adults (parents) of GIV .
•Also in 4 weeks offspring there was highly significant (p<0.005) increase of 13-cell percentage of both GIII b I (diabetic) and GIV b2 (non diabetic).
•The chromosomal anomalies were higher in diabetic animals but this increase was statistically non- significant and within the normal range of abnormalities•In conclusion, the present investigation provided an evidence that hyperglycemic environment surrounding the embryos during their intrauterine period might affect their (3-cell mass rendering them inefficient in producing the required amount of insulin. Administration of vitamin E before induction of diabetes did improve the state of hyperglycemia of diabetic mothers and also the environment that surrounded the fetuses of these mothers and with mild curative effect when administered after diabetes induction in both parents and offspring.