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Abstract This study described spectrophotometric methods for the analysis of thirteen jherapeutically important and widely used fluoroquinolone antimicrobial FQs. These FQs are AMI, CIP hydrochloride, DIF hydrochloride, ENR, GAT, LEV, LOM Lrochloride, NAL, NOR, OFL, PEF mesylate, ROS and SPR. The drawbacks encountered in the analytical methods previously reported in literatures for the analysis of these FQs were behind our interest in selection of these FQs to be the target of this study. The methods developed in the present study include general, selective and specific spectrophotometric methods: 1. General Methods: UHon-Extracttve Ion-Pair Complex formation Method Using SfKH This method describes a generic non-extractive spectrophotometric procedure for determination of all the thirteen FQs by formation of coloured ion-pair complexes with p-naphthol reagent in sulphuric acid medium. The formed ion-pair complexes were measured spectrophotomerically at their corresponding maximum absorption wavelengths. Different variables affecting the reaction conditions were carefully studied and optimized for each particular parameter with the investigated FQs. Under the optimum conditions, linear relationships with good correlation coefficients [0,9987-0.9995] were found between the absorbance readings and the concentrations [ of the FQs in the range of 10-350 ug ml”1 with very small intercepts [-0.0437 to 10.0735]. On the other hand, the precision of the method was satisfactory; the values of relative standard deviations did not exceed 2%. The proposed method was successfully applied to the analysis of the investigated FQs in pure and dosage forms with good accuracy and precisions; the recovery percentages were 97.8 ±0.35 - 102.8 ±1.60%. , The results obtained by the proposed spectrophotometric method were comparable with those obtained by the reference or pharmacopoeial methods. A correlation between logarithm the molar absorptivity [log s] and both the partition coefficient [clog P], heat of formation [HF], minus logarithm HF [pHF], dissociation constants [pK] values and Van der Waal forces [VDW] of the cited FQs |