الفهرس 
Chapter I : IntroductionOnly 14 pages are availabe for public view
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Abstract
Local freshly slaughtered and imported frozen meat available for consumption in Egypt were examined for the occurrence and frequency of clostridial species especially C. perfrengens as one of food poisoning organisms. The bacteriological examination include the direct examination, total viable count, determination of the isolation rates. <Total number of 100 meat samples were examined of which (44) were fresh sample obtained immediately after slaughtering (Egypt meat) and (46) frozen imported meat, Indian (24), Brazil (32. The microscopical examination of clostridium species by Gram’s stain, clostridial species were failed to demonstrate in the films, while the whole culture smears after enrichment for variable periods demonstrated the organisms clearly in all gram’s stained smears as early as 6 hours enrichment.
Total bacterial count:
Fresh meat was found to harbour higher viable count of C. species than the frozen meat, this can be explained due to the effect of freezing and storage in the frozen state on the viability of the spores as well as vegetative cells of the organisms. This can be proved by the results of the isolation rate, where fresh meat showed some what lower isolation rate 45% than the 59%.
Moreover, the incidence of toxigenic strains was nearly the same (50% and 50% in fresh and frozen meat respectively).
C. perfringens type A was the most type frequently isolated it constitute.
The highest rate of C. perfrengens type A (fore and hind quarter) at Egypt 66.66% and equal in Indian and Brazil as frozen meats 33.33%.
The highest rate of C. perfringens type “D” was recorded at Egypt 6 isolates followed by Indian 4 isolate there are no isolates of C. perferngens type “D” at Brazil.
C. perfengens type “A” is the point of significance in this work due to its relation to food poisoning in man 6 (40%.The fresh meat strains harbored high incidence of type “A” 4(66,66%) in Egypt meat than frozen meat 2(33.33%) in (Indian and Brazil meat).
No incidence of harbour of fresh Egypt and frozen meat (Indian, Brazil) to C. perfrengens type “C” and type “D”.
A number of 106 C. perfergens/gm meat will be sufficient to causes food poisoning on consumption of this meat. Comparatively fresh meat (Egyptian bufflue) harbored the highest isolation rate with the isolation of toxogenic strains than frozen meat (Indian buffle- Brazilian cattle). This due to bad manibulation in abittuers and exhusion of the animals before slaughter. Comparatively, frozen meats, Indian 6(30%) meat harbourd C. perfengens higher than Brazil meat (1) 6.6%.