الفهرس | Only 14 pages are availabe for public view |
Abstract In our research, we designed to through somelight on the possibility of characterization, purification of chitosanase enzyme from a high potent procedure, which includes the following steps: 1- Isolation of chitosanolytic actinomycetes from different localities of cultivated soil at Sharkia Governorate. 2- Purification and maintenance of actinomycete isolates. 3- Screening of actinomycete isolates for their chitosanase productivity under shaking conditions. 4- Identification of the most potent chitosanolytic isolates, according to morphological , physiological and cultural properties of isolates. 5- Studying the environmental and nutritional factors affecting chitosanase production by the most potent isolate. 6-Purification of chitosanase(s) produced by the most active organism. 7- Amino acid analysis of the purified-chitosanase(s) enzyme. 8- Studying the factors affecting activity of purified-chitosanase(s) enzyme. 9- Examining the antifungal activity of purified- chitosanase(s) enzyme. |