الفهرس Only 14 pages are availabe for public view
 |  | from | 38 |  |  |
| | | from | 38 | | |
Abstract
- A trial was made to isolate Pateurella multocidia from some chicken farms suffering from respiratory manifestation. In this study a total of 50 random samples were collected from various organs. Incidence of P. multocidia was 48% between the samples.
-Random serological classification showed 4 isolates (A:5, A:8, A:9, D2).
- Sensitivity of the isolated P. multocidia indicated that chloramphenical, danofloxacin and ofloxacin were the most effective antibiotics, the test also reported that pencillin G was the more resistant antibiotic to the isolates.
-Plasmid DNA profile of the isolated P. multocidia by using agarose gel electrophoresis showed different size of plasmid DNA with no obvious relation between plasmid detection and antibiotic sensitivity.
- Polymerase chain reaction (PCR) using 4 random primer was demonstrated to characterize, identify and determine the differences in the DNA sequence at the genome. - RAPD-PCR was used as a key to identify untypable isolates by using the results of typable isolates, which was used as a guide- Only 4 isolates between 16 isolates were gave common band between these isolates and typable one by using primer (3).
- Serological identification of untypable isolates, which not give any result with RAPD-PCR to identify these isolates.12 isolates A:1, 4 isolates A:4 were detected by serological classification (indirect hemagglutination and agar gel precipitation).
Conclusion:
- No obvious relation between presence of plasmid and antibiotic resistance.
RAPD PCR:
-Simple, gave high number of fragments, low cost.
It required purified, high molecular weight DNA, in addition to problem with reproducibility.
-Complete data base for RAPD analysis of individual serotype, it will be easier to detect serotype without the need to lab.