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Abstract
A total of seven Escherichia coli (E. coli) isolates and six Klebsiella isolates were identified biochemically by lactose fermentation, indole, citrate, methyl red, vogues preskauer and lysine decarbolase tests. These isolates were studied for antimicrobial susceptibility patterns by disk diffusion method and micro dillution method. Chromosomal DNA of seven E. coli isolates included in this study was subjected to multiplex PCR with primers specific to blaTEM, blaSHV, and blaOXA genes.
Antimicrobial susceptibility patterns of 15 isolates examined by disk diffusion test, out of 7 E. coli isolates 6 isolates were sensitive to cefotaxime (85.7%), and 5 out of 7 E. coli isolates were sensitive to gentamicin (71.4%). Low sensitivity percentage was detected with enrofloxacin and trimethoprim/sulfamethazol which showed a percent of 42.8% and 14.2% respectively, while amoxicillin, amoxicillin/ clavulanic acid, ampicillin/sulbactam and oxacillin were completely resistant in all E. coli isolates
Antimicrobial susceptibility patterns of 6 Klebsiella isolates were examined by disk diffusion test, and 6 out of 6 Klebsiella isolates were sensitive to cefotaxime (100%), low sensitivity percentage was detected with ampicillin/sulbactam and trimethoprim/sulfamethazol which showed a percent of 16% and 33% respectively, while amoxicillin, amoxicillin/ clavulanic acid and oxacillin were completely resistant in all Klebsiella isolates.