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Abstract
Antimicrobial synergy , resulting from antibiotic combination therapy , is often important in the treatment of serious bacterial infections. So, The aim of this present study to spot the light on the effect of selected cephalosporins and aminoglycoside alone or in combination on P.aeruginosa and C.perfringens and detection the effect of drugs and microorganisms on the haematological and biochemical parameters in infected and treated experimental laboratory animal for selection the most active and safe antibiotic for treatment the infection .
A total of 20 strains were identified biochemically (10 strains of P.aeruginosa and 10 strains of C.perfringens) .These strains were studied in vitro for antimicrobial susceptibility patterns by disk diffusion method, serial dilution method and checkerboard dilution methods.
In vitro, Sensitivity tests revealed that the tested strains of P.aeruginosa were sensitive to cefotaxime, cefepime while it showed a great resistance to cephalexin and cefaclor. So, this study determined MICs for cefotaxime and cefepime alone and each one in combination with amikacin (selected antibiotic from aminoglycoside group).
The vitro results demonstrated that the selected combinations had MICs range lower than MIC of each antibiotic alone. This indicated that combination therapy is more effective in treatment than treatment with an antibiotic alone.
Concerning to the fractional inhibitory concentration index, it was found that cefepime in combination with amikacin , exhibited remarkable synergy with no antagonism results as the fractional inhibitory concentration index was <1.0 as 80% of strains represented synergism and 20 % represented partially synergism indicating the