الفهرس 
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Abstract
Twenty-six bacterial isolates from soil [Egypt (5),cork(4)], cold room air (1), minced beef (5),pork steak (3),room air (1), beef steak (1)and cheese (2) were screened for their proteolytic activities. Vninced beef , park steak and cheese seemed to be good habitats of proteinase-producing bacteria.
Three of the four most efficient proteinase producers, nos 10,18, and 24, were Gram-negative, aerobic, oxidase-positive, case in hydrolyzing,short rods , while the fourth, strain No.20, was an negative oxidase coccus table 2). These four isolated as wellas ps fluorescens Aft 36 and ps. Fluorescens B2No6, from the culture collection at University college, cork , were further assessed for proteinase production using skim milk at 20°c under shaking conditions . ps. Fluorescens aft. 36 was found to be the best proteinase producer isolate no .10 and ps. Fluorescens B2No6 could be used successfully as. Alternatives for ps. Fluorescens AFT 36 asw the proteolytic octivties of tese 3 strain were similar . how ever . ps. Fluorescens AFT 36 was selected to examine the factors that influence roteinase production at 20°c under shaking conditions, since its proteinase has been fairly well studied.