الفهرس 
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Abstract
Cucumber (C. sativus L.), together with melon (Cucumis Melo L.) are the most economically important species in the family of Cucurbitaceae. Genetic variability of these economically important crops is limited by sexual incompatibility with other species in Cucurbitaceae. Thus increase in genetic variation that is frequently associated with tissue culture may prove to be beneficial. Tissue culture may also be used to overcome the sterility barriers by fusing protoplasts of cucumber with protoplasts of sexually incompatible species. Perhaps the most important application of plant biotechnology is the genetic engineering. A major objective of tissue culture research is the development of ability to regenerate whole plants from explants, protoplasts and calli and obtain transgenic plant.
Therefore the present work aimed to :
1- check the response of different Cucurbitaceae species to in vitro culture.
2- Define efficient in two plant regeneration system in two Egyptian commercial hybrids of C . sativus (Amira 2 and Medina)
3- Evaluate somaclones derived from these hybrids.
4- Optimizing the conditions of protoplasts isolation from cucumber cotyledonary leaves.
5- Studying some of the important factors influencing the from the Agrobacterium-mediated transformation in cucumber.