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Abstract
MOHAMED ARMED MOHAMED NAGATY: Regeneration and
transformation of peach (Prunus persica L. Batsch) EL-Sheikh Zewaied
cultivar in North Sinai, Unpublished Doctor of Philosophy, Department
of Plant Production and Protection, Faculty of Environmental
Agricultural Science, Suez Canal University, 2005.
Genetic transformation technique offers the breeders great
opportunity to improve the peach cultivars. In this study the regeneration
frequency and transformation system were established in the local peach
(El-Sheikh Zewaied) cultivar. The embryo survival and regeneration
percentages were 71.0 and 79.8%, respectively. Combination between
BA and IAA was the best constituents for direct somatic embryogenesis.
The Agrobacterium-mediated and microprojectile bombardment
transformation systems were successfully used for introducing the
reporter GUS and selectable bar marker genes in the peach explants under
pressure of 1100 and 1350 psi. Transformation efficiency was lower 3%
by using Agrobacterium. Effect of osmotic and bombardment pressure
treatments on transformation efficiency were tested through a number of
experiments, and positive affects were recorded. The expressions of
introduction of GUS and bar genes were confirmed using PCR analysis,
Southern blotting technique and histochemical assay.