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Abstract
mesophilic isolates) were identified , where the five species were belonged to genus Streptomyces namely; Streptomyces halstedii Salh-12, St. clavus Dakh-28, and St. flaveolus Salh-17 (thermophilic isolates); St. endus Salh-40 and St. albidoflavus Dakh-52 and one identified as Actinomyces aureocirculatus Dakh-50 (mesophilic isolates).
2. The purification of alkaline protease from halotolerant thermophilic St. halstedii Salh-12 and halotolerant mesophilic St. endus Salh-40 were achieved to increase the specific catalytic capacity of the purified enzyme during purification steps. 80% ammonium sulphate was , .