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Abstract
The present study was planned for preparation of
different three types of bacterins corresponding to the rout of
vaccination i.e. formalin inactivated washed P. fluorescens
cells for injection vaccination, formalin-inactivated broth
culture for immersion vaccination and formalin inactivated
wet-packed whole cells for oral vaccination. The humeral
antibody response of vaccinated catfish (C.lazera) was
determined by micro-agglutination test. Moreover efficacy of
the prepared bacterines against infection with P. fluorescens
was detection and calculated as a relative level of protection.
The results revealed that:
1- Catfish (C lazera) immunized by interaperitoneal injection
of formalin-inactivated washed P.fluorescens from first
week post-immunization (5 by Jog2 ) and reached 10 by
log2 at fourth week post-vaccination while catfish
(C lazera) injected interaperilonealy with sterile
physiological saline solution had antibody titer reached 1 by
log2 throughout the experimental period.