الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Commercial dairy farms had serious mastitis problems in spite of its control hygienic measures, for detection of this problem, a total of 3000 and 1500 lactating cows and buffaloes were examined for clinical mastitis in 8 different dairy farms in Ismailia governorate. The results revealed that the incidence of clinical mastitis was 2.6% and 2.1% in examined cows and buffaloes, respectively. 130 milk samples were collected from 108 dairy animals suffering from clinical mastitis. Bacteriological examination performed including isolationand identification of recovered microorganisms. Also polymerase chain reaction (PCR) was conducted to major pathogenic type of isolates. Results revealed that among bacteria isolated were Staphylococcus epidermidis 43.1%, Staphylococcus aureus 26.2%, Streptococcus uberis 6.1%, Bacillus species 3.1% and Enterobacter agglomerans 3.1%. Also negative culture was observed in bacteriological isolation with percentage of 18.5%. Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus uberis were isolated with higher percentage in young age than old age in cows and buffaloes. Staphylococcus aureus and Streptococcus uberis were isolated with high percentage in late lactation in cows and buffaloes but Staphylococcus epidermidis was isolated with high percentage in early lactation in cows and late lactation in buffaloes. The infection in rear quarters was larger than front quarters in cows and buffaloes. Specific oligonucleotide primers were used in a PCR assay to amplify sequences of the nuc gene of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from its genomic DNA. Genomic DNAs extracted from all Staphylococus aureus isolates (n=34) were successfully identified by PCR.
English abstract