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Abstract
The aim of this work is establishment of sensitive methods for
identification of bovine ephemeral fever-Abbassia virus strain (BEF-
A VS). In addition to study of physico-chemical properties of the virus to
determine the best conditions during isolation, preservation, identification
and inactivation of the virus.
For diagnosis ofBEF-AVS, direct fluorescent antibody, indirect ELISA
and virus neutralization tests were carried out.
For study of physico-chemical properties of BEF-A VS, effect of
different treatments were carried out as temperature, PH, cycles of
freezing and thawing, fat solvents, proteolytic enzymes and virus
inactivators,
The results of this study revealed that, direct fluorescent antibody,
indirect ELISA and virus neutralization tests were sensitive methods for
virus identification.
BEF-A VS was sensitive to heat, PH, freezing and thawing cycles (more
than two cycles), fat solvents, proteolytic enzymes, formalin and binary.