الفهرس | Only 14 pages are availabe for public view |
Abstract In this study, three types of vaccines were prepared. K45/20A Brucella oil adjuvenated (Montanide ISA25) vaccine, BEF-BEI inactivated and adjuvenated with (Montanide ISA25) and the third one of vaccine was a combined one which contained both types of organisms (Brucella & BEFV). Each type of vaccine was used in vaccination of a group of calves (four calves in each group with two unvaccinated controls). The humoral immune response against Brucella abortus vaccines was determined by three techniques, rose bengal test (RBT), tube agglutination test (TAT) and complement fixation test (eFT) and by using delayed hypersensitivity test for cellular immunity. On the other hand, the humoral immune response against BEF vaccine was evaluated by serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA) and by using lymphocyte transformation or blastogenesis test for cellular immunity. The results showed that using of Brucella K45/20A-BEF combined vaccine increase the immunity generated when BEF inactivated vaccine is used singly, while the picture was not changed too much when K45/20A vaccine used alone or combined with BEF. Montanide ISA25 is a mineral oil, used as an adjuvant for the first time in the preparation of Br K45/20A vaccine, BEF inactivated vaccine and the combined vaccine. So, it is recommended to vaccinate the Egyptian cattle with the combined vaccine which increase the BEF vaccine immunity with a conserved Brucella immunity, saving effort, time and money. |