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Abstract ~Infection caused by Candida albicans and other related species have increased in prevalence world wide. They range from mucosal d iseases to fatal systemic. episodes in severely neutropenic subjects. Since Candidiasis is almost always opponunisnc, it will recur in many cases j f the llnderlyillg disease is not also treated. Major development in researches into the azole class of ami Itlllgal agents d uring the 1990s have provided expanded options for the treatment of fungal ir1:f~tions as Candida. FulconazoJe has proved to be safer than other antifungal agents. Despite these advances, serious fungal infections remain difficult to treat, and resistance to fluconazole is emerging. In our study We compared the sensitivity of 40 isolates of Candida [18 (45%) strain C. albicans, 12 (30%) C. tropical is, 4(10%) C. glabata, 4(10%) C. krusei and 2(5%) C. parapsilosis] to fulconazole by using broth microdilution adaptation of the National committee for clinical laboratory standards, Etest and Fungitest. Candida species was defined by using germ tube test and CHROM agar. Etcst reading after 24 hrs and 48 hrs proved a highly 1itatistically significant difference in both sensitive, and resistant cases but not in small dose dependent with p. value <0.001, <0.001 and 0.08 respectively. -119- SUMMAR’t AND CO,”iCLUS/UN Results of 24 hrs incubation E test were found to have no significant difference (p value 0.5) when compared with the microdilution method. While the 48hs results had significant difference (P. value <0.01). It W3.’l found that there was no statistically significant difference between Fungitest and microdilution method for all 40 isolates of Candida or for Candida albicans or Candida tropicalis (p. value is 0.9, 0.8, 0.8 respectively). Although both the E test and the Fungitest are promIsmg alternatives tu the NeeLS reference broth microdilution method for susceptibility testing of Candida to flunconazole. Further development is necessary to standardize the medium and incubation conditions, and test procedures before introduction of these as routine methods in the clinical micorbiology laboratory for suscepti bility testing. |