الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
 |  | from | 111 |  |  |
| | | from | 111 | | |
Abstract
B-CLL represent the most common type of adult leukemia
in western societies and is characterized by Absolute
lymphocytosis with painless lymph node enlargement and
prolonged chest infection or pneumonia with accumulation of
anergic, self reactive mature CD5+ B cells in the blood and
expression of CD19, CD20, CD23 and CD27. Also genomic
aberrations can now be identified in approximately 80% of BCLL
patients.
Chemokines are small peptides that are potent activators
and chemoattractants for leukocytes subpopulations and some
non hemopoietic cells. They influence migration of lymphocytes
through a gradient of chemokine concentrations and play a role
in leukocytes adhesion and infiltration during inflammation.
Summary
57
CXCR4 is one member of CXC chemokine family and it
was found that this chemokine receptor together with its natural
ligand (SDF-1) is involved in the regulation of leukocytes
migration and are essential B-lymphopoiesis .
In our study we analyzed expression of chemokine
receptor CXCR4 in B-CLL and its role in migration.
This study included a total of 30 subjects divided into two
groups:
Group I: 20 patients with chronic lymphocytic leukemia
confirmed by laboratory investigations. They include 12 males
and 8 females; their mean age was 61.9 ± 11 years.
Group II: 10 healthy individuals serving as controls with
matched age and sex. They included 6 males and 4 females .
Analysis of the results obtained in the present study was
done by comparative studies between groups I and II as regards
different parameters, and most importantly by correlating these
parameters with the main assayed index.
Summary
55
CXCR4 level in group I and II showed that patients had
higher expression with mean florescence intensity (MFI) than in
control group which show low expression with MFI .
It was found that the expression level of CXCR4 in the
malignant CD 19+ / CD5+ cells was greater than observed in non
malignant B-cells of the peripheral blood .
Also there was significant correlation between the
expression of CXCR4 and peripheral lymphocyte count of BCLL
Cell, and there was significance with CD19, CD23 and
CD5/19 as regarding of % positively of CXCR4 .
Conclusion
CXCR4 can represent a useful diagnostic tool, particularly
in the case of atypical CD5 – negative B-CLL which have no
distinctive phenotypical characteristics.
CXCR4 expression analysis in B-CLL could be of interest.
Moreover, the prognostic relevance of CXCR4 requires further
evaluation. Further insights in the pathophysiology of B-CLL
regarding CXCR4 is highly recommended .