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Abstract
The aims of this work were to: <1- Isolation and partial purification of solubilized crystal toxin proteins from different Bt local isolates and authentic strains and testing their nematicidal activity in controlling root-knot nematode, M. incognita, in vitro.
2- Evaluation the nematicidal activity of the recombinant Cry1A (Cry1Aa, Cry1Ab,and Cry1Ac) toxin protein expressed in E.coli against root-knot nematode, M. incognita, in vitro.
3- Salting out of the vegetative supernatant proteins into separated fractions using ammonium sulfate cut off technique and testing their activity against root-knot nematode, M. incognita, in vitro, then further purification of the highly toxic protein fraction using the Fast Performance Liquid chromatography (FPLC).
4- 2-D electrophoresis analysis for the most active partially purified protein obtained from the FPLC.
5- Detection of the nematicidal genes using specific PCR primers.
6- Screening the nematicidal effects of culture fluid, supernatant and washed pellet of sporulated cells of the most active Bt isolates in controlling root-knot nematode, M. incognita, in vivo.