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Abstract
FMDV is a long known virus but still a current threat in our country with
devastating consequences as shown in FMD outbreak in 2006 in Egypt. One of
the most important FMDV features is the high potential for genetic and
antigenic variation besides the carrier state in FMD, especially in buffalos.
Therefore, molecular study of FMDV is pre-requisite for amplification of
FMDV specific genomic fragment and also copying specific serotypes 0 and A
nucleotides sequence from FMDV variants clinical and cultural samples. Also,
the role of FMDV carrier buffalos in transmitting the virus to in-housed
susceptible livestock should be investigated. - Three buffalo calves were exposed to experimental infection with FMDV OIEGY/93. The 1 st buffalo received bovine derived virus while the 2nd and the 3rd one were inoculated with the virus collected from the preceding infected buffalo. The buffalo calves, after 28 days from the infection of the last buffalo,
were penned with susceptible ruminant farm animals. Also, the virus collected
from bovine was passaged on BHK-21 cells to prepare the vaccine strain.
-FMDV serotype 0 isolation in tissue cultures and demonstration by RT-PCR,
using combination of oligonucleotides, were carried out on various clinical and cultural samples. -In addition, virus isolation and molecular identification of suspected FMDV
during 2006 epidemic in Egypt were performed. Achieved results:
-The experimentally infected buffalo calves showed FMD typical clinical signsHowever, the in-housed livestock with the virus carrier buffalos did not reveal any clinical finding or seroconversion to FMDV. -Virus isolation from FMDV specimens in IB-RS-2 and BHK-21 cells was achieved with a variable sensitivity of the cell cultures. The molecular detection