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Abstract This study was carried out on 269 freshwater fish collected from different localities of freshwater sources (Bohira and Kafr—El— Sheikh Governorates). Fish were 119 apparently • healthy { 6 common carp (CC.), 41 mugils (M.) and 72 catfish (C.F.)J and 150 clinically sick fish (50 from each of C.C.; M. & C.F.). Pathogenicity tests were also conducted in an attempt to evaluate an isolate of A. flavus, Fusarium sp. and a mixed infection of both isolates in 2 experiments. Furthermore, a third experiment was done to determine the LD50 of flavus and Fusarium sp. A fourth experiment was conducted to determine the role of Aflagen in controlling flavus infection in common carp. Results achieved showed that: 1— A total of 816 isolates were recovered from the captured fish. 2— Isolates belonged to 8 fungal genera: \Yeasts, Aspergillus, Fusai’-jum, Penicilijum, Mucor, Cladosporiurn, Alter-noria and Rhizopus. 3— In a. descending fashion, the frequency of isolation was 352 isolates of Yeast (37 from C.C.; 132 from N. and 183 isolates from C.F.); 138 isolates of Aspergillus (29 from C.C.; 42 from M. and 67 from C.F.); 111 isolates of Fusarium (17 from C.C.; 45 from M. & 49 from C.F.); 83 isolates of Penjcj.ijjum (1 from C.C.; 39 from N. & 43 from C.F.). |