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Abstract N albnphine. a ’Yllthelic opioid anal zesic whiell is chemica 11y derived from the oxy norplrone I II ed for pain relief; nowaday ir i addicted by muse aud body builders .. The pre em ’lIldy wa de ugned to 1 reveal which of rhe different toxicological analytical methods i more uitable for the identification of ualbuphine , 1) estimate the postmortem drug concentration Ill’ orue tissues of rats injected intraperitoneally with two different doses of the drua, TIle results obtained call be sunnuarized as follow: I) 0101’ rests revealed that Some chemical reagent’s are suitable for the identification of ualbuphiue (.g. Iarquis Reagent, Frohde’s reazenr and cobalt nitrate I ammonia). 2) Different coloriug reagents were sprayed 01) the chromaroplates of TL and Ul re uns revealed thai iodoplalinate reagent and Dragendorff reagent may be con idered specific for tile identification of na lbuphlne _ A,3) TIll! chromatographic beha ior of the dmg 011 silica gel F254) is considered impcrrnnr for it identification. There were different calculated Rf value with different eluent’s, 4) The U spectnun of nalbuphine showed ab orption baud at). 1Ilil’ 248 um, 5 HPL chrcmatogran cf’nalbuphiae demonstrated that retention time (R1)was ~.33 utili Infrared pectl”oscopy anal)’ i of nalbuphine demonstrated it cbaracterisri fuuction g[Oups of ualbupluue. 7 Till ’n- NMR spectnnu of ualbuphiue dis olved in chloroform \MfI carried out to confirm the protons pre I’ll! in its stru ture, 8 TIle characteristic iua pecnum of rhe dms wa identified and the mil s fragments with their assigned structure were defined, 9 HH: quantitative estimation of the drug in different or@an ohms b HPLC were suece sfully carried out Calibration curve w plotted usine different concentrations of nalbuphine aeainsr the area under PDF created with pdfFactQry Pro trial version YNJW.Rdffaclory,com Genera I. IjllJJNI!)’ Peaks, and cousequemly the concentration of nallmpbine residue in differeut ti ne and blood were obtained. lO) lalbuphil’l~ distribntion of in different org<1IlS of rats as well a their abundance per gmlll Ii sue Was illu usted. Blood, pleeu, hair, brain tissu were found to tlle organs of choice for toxicological analy is regardless to the dose level. |