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العنوان
Hormonal Induction of Spawning In African Catfish Clarias Iazera =
الناشر
Ismail Ismail Yousef Abo-Ghanema ,
المؤلف
Abo-Ghanema, Ismail Ismail Yousef
هيئة الاعداد
مشرف / سمير عبد المجيد غنام
مشرف / بهيج رياض نعمه الله
مناقش / على عبد المقصود فرحات
مناقش / عبد الدايم زكريا محمد
باحث / اسماعيل اسماعيل أبو غنيمة
الموضوع
African catfish - Spawning - Hormonal induction. Animal Physiology .
تاريخ النشر
1999 .
عدد الصفحات
81 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
الناشر
Ismail Ismail Yousef Abo-Ghanema ,
تاريخ الإجازة
27/07/1999
مكان الإجازة
جامعة الاسكندريه - كلية الطب البيطرى - فسيولوجيا الحيوان
الفهرس
Only 14 pages are availabe for public view

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from 79

Abstract

One of the most serious problems encountered in fish farming is the difficulty of obtaining fry in large numbers for catfish breeding programs on commercial scale. The main problems include:
1- Catfish oocytes have sticky layer which adhere them together thus preventing proper aeration and lower egg hatchability.
2- The need of obtaining large number of mature oocytes whether through induction of spawning or through in vitro maturation and their subsequent fertilization with proper amount of milt to obtain large number of fry suitable for intensive catfish farming. The present study was undertaken to study the possible use of some hormones to induce oocyte maturation in vitro and to find a suitable method to overcome the problem of egg adherence.
The catfish breeding programs , as well as the difficulty of obtaining seed of the most important problems facing aquaculture for those types of fish on the economic scale to produce large numbers of seeds , as well as the most important of these problems :
1 - get a sufficient quantity of semen from the testicle .
2 - those fish eggs covered with a sticky layer , which reduces the rate and hatching eggs are not subjected to any of the transactions.
3 - rotting eggs and not فقسة after the incubation process .
But this has been such a study to discuss how to solve problems and get the highest rate for the production of seed for this kind of fish.
The study also included the impact of certain hormones on the degree of maturity of the eggs outside the body and determine the best .
* First experiment :
Ninety was used female catfish for ovarian After you determine the weight to the nearest gram and then use the two parts of each fish ovary .
To conduct the experiment factors first part Bgamsh in a solution shown to determine the degree of maturity of the eggs and the second part was used to study the effect of various hormones which were processed eggs enzyme Alvakimuterbsin order to remove the material Allazjh and not Altsalq eggs were incubated eggs with hCG for 6 hours in a solution of Aarles department eggs into nine groups, each group were incubated for 24 hours in a solution of Earls .
where the use of three effects of each hormone for each one millimeter from Earls solution after a period of incubation with various hormones laying eggs in a solution of clarification and examination by anatomical to determine the degree of maturity of the eggs in each group.
The results showed the following :
Led those hormones such concentrations to a significant increase in the size and maturity of the eggs .
* The second experiment :
Fifty was used male catfish as well as fifty females where injected intramuscularly Bmsthalb the pituitary gland to the thickness of the carp .
After a period of 18-21 hours from the beginning of the injection examined females to test ovulation massage the abdomen where the exit eggs from the slot genital fish painted greenish brown evidence of ovulation in the case and before getting the eggs from the female catfish massage the abdomen were slaughtered male catfish and getting testicular and squeeze in a saline solution 0.9 % and then were collected eggs in petri dishes and then distributed solution that contains sperm on eggs and stirring the solution and eggs gently to an enrichment and then transfer the fertilized egg and the bosom of the temperature of the room where the processed eggs to remove gunk from eggs to prevent التصاقة and not to hinder the eggs to hatch .
* Experience has shown the following results :
1 - emulsion injection pituitary gland to the thickness of the female catfish carp led to egg maturation and ovulation occurs in each of the injected fish .
2 - also led to an increase in the size of the testis in males and increase the amount of semen .
* And draws the following results :
1 - the most influential of synthetic hormones in the events of ripening eggs in Fish catfish out of the body are:
- Emulsion pituitary gland to carp led to the maturity of females and males Fish catfish which encourages use it instead of other treatments due to the availability and ease of getting it .