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Abstract - This study was carried out to establish the prevalence of some bacterial and fungal diseases in chickens, eggs, and chicken environment and to assess the possible role that played by chickens in transmitting some human pathogens. - A total of 1280 samples were collected from living chickens, chicken environment, eggs, and human beings. 360 cloacal swabs were collected from living chickens including 200 swabs from layers, and 160 swabs from ‘otot. Sct o thkc’&tt tNVLWLtV& (‘cc NckcX kvc \ sampf’es, !d?? Eêea’ sampr rra’ c rsrmpk. ig x-rrfrdrs included 100 samples from eggshell and 60 samples from egg Conten9ts. While, samples of human beings (160) were collected from swabs and stool of some individual whom their occupation necessitate their contact with chickens and their environmeM. Another 160 nasal and tracheal swabs (from chickens) and 40 sputum samples (from farm• workers) were collected. These samples were collected from fattening and laying farms in Kafr EI-Sheikh province throughout the four seasons (summer, autumn, winter and spring). - Samples were prepared and examined bacteriologically and mycologically for isolation and identification of pathogenic and potentially pathogenic bacteria and fungi. The obtained results showed the following: - Several pathogenic and potentially pathogenic bacteria were isolated from living chickens, environment, eggs, and human beings. Types and incidence of these pathogens was represented as the following: E. coli sp. (2.78, 2.75, 1.25 and 5.63%); Salmonella sp. (2.22, 2.25, 2.5 and 3.75%); Shigella sp. (3.33, 3.75, 6.88 and 6.88%); Klebsiella sp. (0.0, 0.25, |