الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 219 |  |  |
| | | from | 219 | | |
Abstract
The present work is concerned with the effect of caffeine on
the prenatal embryo of mice. In addition other parameters were also
studied. Caffeine is an active nervous system stimulant that is
consumed daily by a large segment of human as coffee, tea and cola.
The present work is concerned with the effect of caffeine on
the prenatal embryo of mice. In addition other parameters were also
studied. Caffeine is an active nervous system stimulant that is
consumed daily by a large segment of human as coffee, tea and cola.
Caffeine has been shown to cross the placental and reach the foetus.All groups were subcutaneously injected with a dose of
caffeine ”18 mglkg body wt,”.
The present iwesligatlon Includes the following embryos:
Preimplantation blastocyst ”81 h.p.c”, true gastrula ”105
h.p.c.’~&J1day embryo ”129 h.p.c”, 8th day embryo ”177 h.p.c” and 20th
day of gestation. As well as postnatal viability of the youngs ”from In
to 14th day after birth”were examined.
Morphologically, caffeine treatment causes the following effects:
- Increase death rate in mice.
- Decrease in the percentage of mating in females. However, in males ’’’’
the percentage of mating is not affected.
- Decrease the rate values of body weight in mice.
- Increase in the percentage of abortion.
- Decrease in maternal uterine weights.
- Increase in the percentage of foetal mortality ”resorptions and
stillbirths” at 20th day of gestation”. As well as, caffeine increase in
the percentage of mortality of offspring through two weeks after
delivery, Offspring of the In experimental group, showed no death
through the 2nd week.
- Decrease in lengths and weights of foetuses at the 20th day of
gestation and offspring after delivery.- Produce some deformed prenatal embryos at 20th day of gestation’
- Appearance of dark red patches ”haematoma” at different parts of
the body”at 20th day ofgestatiorl’.
The ovaries and testes of females and males treated with caffeine for 21 days before
mating” in the case of no detection implantation site” showed histopathological effects:
- The ovaries showed congestion blood vessels, haemorrhage
degeneration of some follicles and reduction in the number of the
Graafian follicles and corpora lutea.
- The testis showed cellular debris inside the lumen of some
seminiferous tubules and undifferentiation between various types of
spermatogenic cells.
The skeletal system of the parentally treated foetuses with caffeine at the 201!!day of
gestation showed:
- Growth retardation of some bones represents by shortness of ribs
and decrease in length of fore & hind limbs and pectoral & pelvic
girdles.
- Severe lack of ossification in some others ”bones of the skull, some
vertebrae, sternum, pubis and phalanges of fore & hind limbs”.Histological examination of the embryos ”4th, sJ.b , fj1Il and alb day of pregnancy
Parentally treated with caffeine” G2, G3 and G4 ”Showed delay In growth. However,
embryos of the 1.11experlmel’llal group ”G1
” showed unremarkable changes:
- The cells of the inner cell mass and trophoblast of the blastocysts
maternally treated with caffeine ”G2” are not differentiated. While
the inner cell mass of the blastocysts parentally treated with
caffeine ”G3” and ”G4” not completely developed.
- The true gastrulae maternally treated with caffeine ”G2
” showed
slight delay in growth ”distal and proximal endoderm not
developed”. The same embryos parentally treated with caffeine ”G
3
and G4” are found in the blastocyst stages.
- The E)th. day embryos parentally treated with caffeine”G2
,G3and G
4
”
Showeda considerable delay in their growth; the embryos resemble
true gastrulae with no distal endoderm.
- The embryos at the 8th day of pregnancy parentally treated with
caffeine showed a considerable delay in their growth ; the
ectoplacental cone is less develoved”G2, G3 and G4
” and the chorion
and amnion not- completely developed”G3and G4
”.
The foetuses at the 2()1Il day of gestation parentally treated with caffeine showed
pronouced hIsIopathologJcaJeffects in the fiver, kidneys, lungs and eyes:
- The liver showed venous congestion, hydropic & fatty degenerations,
fatty infiltration, necrosis and lymphocyticinfiltration.• The histopathological changes in the kidney are manifested by
glomerulonephritis of the Malpighian corpuscles, cloudy swelling,
hydropic degeneration and necrosis.
- The lung showed congestion blood vessels, pulmonary oedema, cell
detachment of the terminal and respiratory bronchioles and
alveolar ducts.
- The eye lesions included folding and undifferentiation of the retinal
layers, reduced lens size and adhesion of the cornea and eyelid.