Search In this Thesis
   Search In this Thesis  
العنوان
Evaluation of methods used for diagnosis of Giardiasis in children/
الناشر
Ain Shams University. Faculty of medicine. Department of pediatrics.
المؤلف
Tahoun,Mahmoud Mahmoud Mostafa
هيئة الاعداد
باحث / Mahmoud Mahmoud Mostafa Tahoun
مشرف / Zienab Anwar Elkabbany
مشرف / Amel Abdel Mageed Elfaramawy
مشرف / Eman Abdel Moniem El Gohary
تاريخ النشر
2008 .
عدد الصفحات
116P.
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
طب الأطفال ، الفترة المحيطة بالولادة وصحة الطفل
تاريخ الإجازة
1/4/2008
مكان الإجازة
جامعة عين شمس - كلية الطب - pediatrics
الفهرس
Only 14 pages are availabe for public view

from 134

from 134

Abstract

Giardia lamblia is a widely distributed flagellated protozoan parasite that inhabits the small intestine . It is the most prominent human protzoal enteropathogen with a prevalence rate that varies from 2% to 5% in the industrialized world and 20 % to 30% in the developing world . Giardiasis is more prevalent in infancy and children .
Diagnosis of giardiasis is largely dependent on microscopic demonstration of the parasite in the stool, which yields many false negatives due to intermittent excretion of Giardia lamblia . Another reliable methods of diagnosis are duodenal aspiration and intestinal biopsy but they are invasive and uncomfortable techniques . So, some other diagnosis techniques were introduced and were studied regarding to their value in diagnosis but these studies were not fulfilled up till now especially regarding to their value as methods in cure assessment .
The aim of this work is to evaluate the use of ELISA for detection of Giardia lamblia antigen in the stool, as a diagnostic technique of giardiasis and to compare between this method and the conventional microscopic examination .
This study was conducted on 80 children who were chosen from Sharkya Government (Belbis and Mashtoul cities) they were :-
1)- Group I (Cases) : they were 55 cases complaining from gastrointestinal symptoms suggestive of giardiasis including abdominal pain , diarrhea , anorexia , fatigue, nausea&vomiting and loss of weight .
2)- Group II (Controls) : they were 25 controls not complaining from gastrointestinal symptoms suggestive of giardiasis .
All studeid individuals were subjected to history taking , clinical examination , microscopic stool examination and complete blood count (CBC) . All stool samples were then subjected to ELISA testing for immunological detection of Giardia lamblia coproantigen .