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Abstract According to the practical significance, thawing and post-thawing fmdings of frozen buffalo-bull and bull spennatowa has been given consideration in this investigation. A series of experiments were conducted to investigate: The effects on sperm motility, alive percentage and acrosome integrity, under different thawing rates. (Experiment 1). The effects on sperm motility, alive percentage and acrosome integrity, under different post-thawing rates. (Experiment 2,3). The effects of post thaw holding of straws in the thaw bath on various post thawing temperatures to examine sperm motility, alive percentage and acrosome integrity (group or stock thawing) (Experiment 4). In all experiments the results were evaluated by determination of the post- thaw sperm motility (visual estimation), alive percentage by means of vital staining with Eocin-Negrosine staining and acrosome membrane integrity by means of silver nitrate staining. Experiment 1 Buffalo-bull and bull spermatozoa processed in straws 0.5 ml French straws of buffalo-bull straws and 025m1 Continental bull straws was thawed at 12/35°C and post thawed at once, 60/10°C, 60t20°C, 60/30°C and 60/40°C(control group). Examination of semen was performed immediately after thawing at once as well as at 30 seconds for post thaw time, to detect the significance in the following parameters: sperm motility, alive percentage and acrosome membrane integrity. |