الفهرس 
ملخص الرسالة
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Abstract
A and potent inactivated alum adjuvant vaccine against RVF was locally prepared.
The virus was propagated in BilK monolayer culture at and M.O.I. between 0.1-0.01. The combination adsorption, no pouring off with serum wa te adopted technique as it gave the highest virus titer. RVF propagation in BilK cells showed three phases concerning the total multiplicity output, a sho’t lag phase, then a growth phase and -finally a long station%phase. During this stationary phase there was .destructio of the cell sheet and the virus could be harvested reaching its peak titer by the 48th hour post—infection.
Although the virus multiplies in the cytoplasm of the ecteci cells, yet there might be an
intranuclear phase as onstrated by the
fluorescence of the nucleus during virus wth. Suckling mice were used for virus titration especially e vaccine virusfr the repioductibility of the results. vertheless adult mice may be used taking in consideration