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Abstract
Respiratory diseases constitute a serious problem to rearing of both dairy and beef cattle all over the world. Now, BRSV is considered an economic important respiratory tract pathogen, causing serious losses in calves and adult cattle. Therefore, the present study was conducted to isolate and identify the BRSV in cattle and buffalo showing respiratory manifestations in the form of nasal discharge, coughing, respiratory distress and elevation of body temperature.
In the present study, a total of 180 (129 nasal swab and 51 lung tissue) samples were collected from both imported and native cattle and buffalo from different localities of Sharkia Governorate. Only 88 out of 180 samples were screened by sandwich ELISA commercial kits for detecting BRSV antigen. Only one lung tissue sample was positive obtained from a buffalo with a percentage of 1.1 %.